The Complete Microbiome Mapping Test is an advanced diagnostic tool designed to analyse and provide a comprehensive understanding of an individual's gut microbiome. The gut microbiome refers to the diverse community of microorganisms that lives in the gut. This cutting-edge test uses state-of-the-art sequencing technologies to examine the genetic material of the microbial population, providing insights into the composition and function of the gut microbiome.
The large intestine is packed with bacteria, whilst the small intestine typically contains very few bacteria. If the small intestine becomes overgrown with bacteria it can lead to malabsorption of key nutrients, causing symptoms such as nausea, vomiting, diarrhoea, bloating, fatigue, malabsorption of nutrients and weight loss. Small Intestinal Bacterial Overgrowth (SIBO) is also thought to be a possible factor in some cases of fibromyalgia and chronic fatigue syndrome.
A food intolerance response takes place in the gut, and it occurs when you are unable to properly breakdown the food. This could be due to enzyme deficiencies, sensitivity to food additives or reactions to naturally occurring chemicals in foods. Often people can eat small amounts of the food without causing problems.
The Food Intolerance Check assesses your response to 96 foods. Your blood is screened for IgG antibodies to these foods using the ELISA method.
This is a finger prick blood spot test that doesn't require a visit to a collection centre.
Note that The Australasian Society of Clinical Immunology and Allergy (ASCIA) does not recommend IgG food intolerance testing as a diagnostic tool for food-related symptoms. According to ASCIA, the presence of IgG antibodies to specific foods does not prove the existence of a clinical intolerance or sensitivity. Clinical evaluation, medical history, and elimination diets are the preferred methods for identifying food intolerances or sensitivities.
The lining of the intestine and gut microbiome make the gut highly selective in absorbing specific nutrients. When this gut lining is compromised, unwanted substances can cross the barrier and activate an immune response. The test works on the principle that small molecules are readily absorbed by the gut and excreted in the urine, whilst larger molecules are not. Under normal circumstances the ratio of large molecules to small molecules is low. Where ‘leaky gut’ is present, the larger molecules are found to penetrate the gut lining and are detected in the urine.
- Note this test CANNOT BE PERFORMED IF YOU ARE A DIABETIC with a fasting urine glucose level over 6mmol/L.
What we test
Food Intolerances
This Food Intolerance Check includes 96 IgG intolerances to the following: Dairy: Casein, Cheddar Cheese, Cow's Milk, Goat's Milk, Whey. Fish/Shellfish: Clam, Cod, Crab, Halibut, Lobster, Salmon, Scallop, Shrimp, Sole, Trout, Tuna Grains/Legumes/Nuts: Almond, Barley, Buckwheat, Chestnut, Chickpea, Coconut, Corn, Gliadin, Wheat, Gluten, Wheat, Green Bean, Green Pea, Hazelnut, Kidney Bean, Lentil, Lima Bean, Oat, Peanut, Pecan, Pinto Bean, Rye, Soybean, Spelt, Walnut, Wheat, Whole, White Rice Fruits: Apple, Apricot, Banana, Blueberry, Cranberry, Grapefruit, Grape, Lemon, Olive, Orange, Papaya, Peach, Pear, Pineapple, Plum, Raspberry, Strawberry Meat/Poultry: Beef, Chicken, Lamb, Pork, Turkey Egg: Egg White, Chicken, Egg Yolk, Chicken, Egg Whole, Duck Misc/Herbs/Spices: Baker's / Brewer's Yeast, Button Mushroom, Cane Sugar, Cocoa Bean (Chocolate), Coffee Bean, Flaxseed, Ginger, Honey, Oregano, Sesame Seed, Sunflower Seed Vegetables: Asparagus, Avocado, Beet, Broccoli / Brussel Sprouts, Cabbage, Carrot, Cauliflower, Celery, Cucumber, Garlic, Green Bell Pepper, Lettuce, Onion, Pumpkin, Spinach, Sweet Potato, Tomato, White Potato Candida Screen: Candida Albicans
Intestinal Permeability (Leaky Gut)
This urine test assesses levels of a synthetic sugar and mannitol that can be used to monitor improvements or deterioration in intestinal permeability.
Lactulose is a 'large' molecule, and only small levels should be absorbed by the gut and excreted into the urine.
Mannitol is a 'small' molecule which is readily absorbed by the gut and subsequently excreted into the urine.
Where 'leaky gut’ is present, the large synthetic sugar molecules can penetrate the gut lining and are recovered in the urine, resulting in a high recovery ratio.
SIBO Lactulose Breath Test
The lactulose test is used to measure oro-caecal transit time (OCTT) - this is the time interval between ingestion of lactulose and rise in breath hydrogen. Prolonged OCTT has been associated with SIBO.
Bacteria in the small intestine produce hydrogen and methane gas when exposed to lactulose. Hydrogen excretion is indicated by >20ppm sustained rise upon lactulose challenge.
Bacteria in the small intestine produce hydrogen and methane gas when exposed to lactulose. Methane excretion is indicated by >10ppm sustained rise upon lactulose challenge.
Normal OCTT (the time it takes for lactulose to pass through the gut) in healthy adults is approx 60-120min.
Faecal Macroscopy
Macroscopy looks at stool colour and formation, as well as for evidence of mucous or blood which may require further investigation.
Brown is the colour of normal stool. Other colours may indicate abnormal gastrointestinal conditions.
A formed stool is considered normal. Variations to this may indicate abnormal gastrointestinal conditions.
Mucous production may indcate the presence of an infection, inflammation or malignancy.
This test is used to detect bleeding in the digestive tract, and is used in Australia's National Bowel Screening program. This test can detect tiny traces of blood in the stool, and can indicate the presence of disease at a relatively early stage when stools may appear normal.
Short Chain Fatty Acids
Short-chain fatty acids (SCFAs) are produced by gut bacteria through the fermentation of dietary fibre. They have numerous benefits for gut health, including serving as an energy source for colon cells, maintaining the gut barrier function, reducing inflammation, regulating appetite, modulating the immune response, and protecting against pathogens.
Short-chain fatty acids (SCFAs) are produced by gut bacteria through the fermentation of dietary fibre. They have numerous benefits for gut health, including serving as an energy source for colon cells, maintaining the gut barrier function, reducing inflammation, regulating appetite, modulating the immune response, and protecting against pathogens.
Decreased Butyrate levels may indicate inadequate colonic function.
Decreased Acetate levels may indicate inadequate colonic function.
Decreased Propionate levels may indicate inadequate colonic function.
Valerate is a type of short-chain fatty acid produced by certain gut bacteria through the fermentation of dietary fibres. Valerate plays and important roles in maintaining gut health and overall well-being.
Gastrointestinal Functional Markers
This protein is released into the stool when the intestinal lining is damaged. It's usually very high in cases of Inflammatory Bowel Disease (such as Crohn's or ulcerative colitis), and low in IBS. Calprotectin is also elevated with gut infections and colon cancers.
Pancreatic Elastase is used to assess pancreatic exocrine function and levels reflect the activity of the pancreatic enzymes trypsin, chymotrypsin, amylase and lipase.
Faecal secretory IgA (sIgA) is secreted by mucosal tissue and represents the first line of defence of the GI mucosa and is central to the normal function of the GI tract as an immune barrier.
Zonulin is a protein that facilitates the opening of tight junctions between cells of the wall of the intestinal lining to allow for passage of nutrients and fluids into the body.
b-glucuronidase is an enzyme that breaks the tight bond between glucuronic acid and toxins in the intestines. The binding of toxins in the gut is protective by way of blocking their absorption and facilitating excretion.
The steatocrit is a measure of faecal fat excretion and is associated with pancreatic insufficiency.
The tTg antibodies test is the most sensitive and specific gluten intolerance test for coeliac disease. This tTG test can also be used to help evaluate the effectiveness of treatment as antibody levels should fall when gluten is removed from the diet.
Imbalances in gut pH influence short chain fatty acid production and their effect.
Key Microbiota
The two largest phyla making up the gut microbiome in humans are Firmicutes and Bacteroidetes. The relationship of these two large groups, expressed as the Firmicutes/Bacteroidetes ratio, has been associated with a number of pathological conditions.
Studies have shown that when there is a higher ratio of Firmicutes within the gastrointestinal tract, that there is a link to obesity.
Bacteroidetes are bacteria that ferment polysaccharides and otherwise indigestible carbohydrates and produce short-chain fatty acids (SCFAs) that have many beneficial effects in the gut.
Firmicutes bacteria make up the largest part of the gut microbiome. Due to their negative influence on glucose and fat metabolism, they are commonly referred to as bad gut microbes.
Parasites
Some gut problems can be a consequence of an undetected gut infection. A significant number of IBS (irritable bowel syndrome) cases could actually be due to undiagnosed gut parasites such as Blastocystis hominis and Dientamoeba fragilis.
Cryptosporidium is a microscopic parasite that causes the diarrhoeal disease cryptosporidiosis. Both the parasite and the disease are commonly known as "Crypto."
Amoebiasis is a parasitic disease (also known as amoebic dysentery) caused by infection with Entamoeba histolytica or another amoeba (for example, E. dispar). The disease may not cause symptoms in most individuals.
Symptoms of Giardia infection can occur with 3 to 25 days and may include diarrhoea, nausea, vomiting, pale greasy foul-smelling stools, stomach cramps, passing excess gas, bloating, weight loss and fatigue.
Some research suggests that people with IBS may be more likely to have Blastocystis hominis organisms in their stool.
The bacteria can be present in the gut for months or year and misdiagnosed as IBS. Infection can cause diarrhoea, abdominal pain and cramping, anal itching, nausea, loss of appetite, headache, dizziness, depression, weight loss and fatigue.
Endolimax nana transmission occurs by ingesting contaminated food or water. Infections may be no symptoms or present with diarrhoea.
Entamoeba coli is a non-pathogenic amoeba that commonly lives in the human gastrointestinal tract without causing harm or illness.
Pentatrichomonas hominis is a non-pathogenic parasite. While it may be associated with mild gastrointestinal symptoms, it is generally does not require specific treatment unless symptoms are severe or persistent.
Worms
PCR-based stool worm tests are particularly useful when other diagnostic methods, such as microscopic examination of stool samples, do not yield definitive results. They can provide a more precise identification of the specific worm species present, aiding in targeted treatment and management of an infection.
Opportunistic Bacteria
In a healthy individual with a balanced gut microbiome, opportunistic bacteria are usually kept in check by the beneficial bacteria that dominate the gut ecosystem. However, when the microbial balance is disrupted or the immune system is weakened, these opportunistic bacteria can multiply and potentially lead to infection or other health issues.
B. licheniformis and B. subtilis are associated with food-borne diarrhoeal illness and meat dishes are a common source of infection.
Enterococcus species are part of normal flora in the human gut, but can however be implicated in a variety of infections of which urinary tract infections are the most common.
Enterococcus species are part of normal flora in the human gut, but can however be implicated in a variety of infections of which urinary tract infections are the most common.
M. morganii originates from the gill and skin of fish. It is possible that it may cross-contaminate during handling of fish in processing plants and restaurants. Diarrhoea has been associated with infection of this organism
Pseudomonas is found in water and soil as well as fruits and vegetables, and is considered an opportunistic pathogen.
Pseudomonas is found in water and soil as well as fruits and vegetables, and is considered an opportunistic pathogen.
Food poisoning is often attributed to staphylococcus. Symptoms of staphylococcal food poisoning usually appear within 1 to 6 hours after ingestion but depends upon the amount of contaminated food eaten. Nausea, vomiting, abdominal cramping, and diarrhoea are the most common symptoms. Recovery generally takes two day, but can sometimes take longer.
Food poisoning is often attributed to staphylococcus. Symptoms of staphylococcal food poisoning usually appear within 1 to 6 hours after ingestion but depends upon the amount of contaminated food eaten. Nausea, vomiting, abdominal cramping, and diarrhoea are the most common symptoms. Recovery generally takes two day, but can sometimes take longer.
Streptococcus is common in the gut flora. With the exception of very rare cases, streptococcus species are not implicated in gastric disease.
Streptococcus is common in the gut flora. With the exception of very rare cases, streptococcus species are not implicated in gastric disease.
Streptococcus is common in the gut flora. With the exception of very rare cases, streptococcus species are not implicated in gastric disease.
Streptococcus is common in the gut flora. With the exception of very rare cases, streptococcus species are not implicated in gastric disease.
Streptococcus is common in the gut flora. With the exception of very rare cases, streptococcus species are not implicated in gastric disease.
Methanobacteriaceae are microbes that produce methane. Facilitates carbohydrate fermentation and short-chain fatty acid production by beneficial bacteria.
This bacteria has been implicated in gastrointestinal disorders such as ulcerative colitis and inflammatory bowel disease.
Enterobacter complex are opportunistic pathogens that may be responsible for a wide variety of infections, and are present in the environment (in soil and sewage) and in the gut flora of humans.
Potential Autoimmune Triggers
An imbalance in the gut microbiome (called ‘dysbiosis’) has been associated with autoimmune diseases, particularly, rheumatoid arthritis, type 1 diabetes, multiple sclerosis and autoimmune liver disease.
Citrobacter is considered an opportunistic pathogen and therefore can be found in the gut as normal flora. It is occasionally implicated in diarrhoeal disease, particularly C. freundii, C. diversus and C. koseri.
Citrobacter is considered an opportunistic pathogen and therefore can be found in the gut as normal flora. It is occasionally implicated in diarrhoeal disease, particularly C. freundii, C. diversus and C. koseri.
Klebsiella forms part of the normal gut flora in small numbers, but can be an opportunistic pathogen.
Klebsiella forms part of the normal gut flora in small numbers, but can be an opportunistic pathogen.
Some studies have suggested associations between Prevotella copri and certain health conditions, while other studies have indicated potential beneficial roles.
Part of the normal flora of the gastrointestinal tract, though has been shown to be an independent causative agent of intestinal disorders. May also play a role as an opportunistic organism in enteric infection due to other pathogens. Food has been implicated as a vehicle of infection.
While Proteus mirabilis is a natural inhabitant of the gut and doesn't usually cause problems in healthy individuals, it is known to be an opportunistic pathogen and can cause infections under certain circumstances.
Fusobacterium bacteria is commonly found in the mouth and the gut. In the mouth, high levels are strongly linked to oral hygiene. In the gut, high levels have been observed in individuals with colon cancer and appendicitis.
Fungi & Yeast
This test looks for evidence of candida or other yeast overgrowth. Whilst yeasts are a normal inhabitant of the gastrointestinal tract, they may become an opportunistic pathogen after disruption of the mucosal barrier, imbalance of the normal intestinal flora or impaired immunity. This can be caused by things like antibiotics, antacids and stress.
Candida is a type of yeast that grows in the body in areas such as the mouth, gut and vagina. At normal levels, it does not cause any problems, but when a person has an overgrowth of Candida in the gut, it can appear in stools.
Candida is a type of yeast that grows in the body in areas such as the mouth, gut and vagina. At normal levels, it does not cause any problems, but when a person has an overgrowth of Candida in the gut, it can appear in stools.
Candida is a type of yeast that grows in the body in areas such as the mouth, gut and vagina. At normal levels, it does not cause any problems, but when a person has an overgrowth of Candida in the gut, it can appear in stools.
Candida is a type of yeast that grows in the body in areas such as the mouth, gut and vagina. At normal levels, it does not cause any problems, but when a person has an overgrowth of Candida in the gut, it can appear in stools.
Candida is a type of yeast that grows in the body in areas such as the mouth, gut and vagina. At normal levels, it does not cause any problems, but when a person has an overgrowth of Candida in the gut, it can appear in stools.
Candida is a type of yeast that grows in the body in areas such as the mouth, gut and vagina. At normal levels, it does not cause any problems, but when a person has an overgrowth of Candida in the gut, it can appear in stools.
Candida is a type of yeast that grows in the body in areas such as the mouth, gut and vagina. At normal levels, it does not cause any problems, but when a person has an overgrowth of Candida in the gut, it can appear in stools.
Candida albicans is the main type of yeast which colonises the human body. It normally lives in the gastrointestinal tract and other areas of the body without causing problems, but imbalance in the microbiome can lead to overgrowth.
Candida is a type of yeast that grows in the body in areas such as the mouth, gut and vagina. At normal levels, it does not cause any problems, but when a person has an overgrowth of Candida in the gut, it can appear in stools.
Candida is a type of yeast that grows in the body in areas such as the mouth, gut and vagina. At normal levels, it does not cause any problems, but when a person has an overgrowth of Candida in the gut, it can appear in stools.
Candida is a type of yeast that grows in the body in areas such as the mouth, gut and vagina. At normal levels, it does not cause any problems, but when a person has an overgrowth of Candida in the gut, it can appear in stools.
Geotrichum yeasts can be found in soil, dairy products and in human skin and mucosae, and are usually only considered an opportunistic pathogen in immune-compromised hosts. Geotrichum may play a role in IBS.
A common environmental yeast which is not considered pathogenic in nature. These yeasts are ubiquitous in the environment and can be found on fruits, vegetables and other plant materials.
S. cerevisiae is commonly known as Bakers or Brewers yeast. It commonly colonises mucosal surfaces but isn't considered an opportunistic pathogen. Overgrowth may be associated with dietary ingestion of as part of a "health food" regimen.
Bacterial Pathogens
This panel also looks for evidence of other bacteria which may be pathogenic in nature, such as C.Diff, Citrobacter and Klebsiella.
Aeromonas are bacteria that can cause an acute diarrhoeal illness that normally clears without treatment. It is a fairly common cause of gastroenteritis, which occurs most often throughout the warm summer months in most countries.
Campylobacter infection (campylobacteriosis) is a bacterial infection which most commonly causes gastroenteritis (also known as 'gastro') but may also cause illness affecting the entire body.
Clostridium difficile, also known as C. difficile or C. diff, is bacteria that can infect the bowel and cause diarrhoea. The infection most commonly affects people who have recently been treated with antibiotics. It can spread easily to others.
Clostridium difficile, also known as C. difficile or C. diff, is bacteria that can infect the bowel and cause diarrhoea. The infection most commonly affects people who have recently been treated with antibiotics. It can spread easily to others.
Enteroaggregative E. coli (EAEC) is a type of bacteria that can cause intestinal infections. It's one of several strains of E. coli, and it's known for causing diarrhoea, particularly in developing countries and among travelers.
Enteropathogenic E. coli (EPEC) is a type of bacteria that can cause severe diarrhea, particularly in young children. It's one of the most common causes of diarrhoea in developing countries.
E. coli O157 is a specific strain of Escherichia coli bacteria that can cause severe foodborne illness. It's particularly dangerous because it produces a toxin that damages the lining of the intestines.
Hypervirulent Clostridium difficile (HCD) is a strain of the bacteria Clostridium difficile that is more virulent and resistant to antibiotics than traditional strains.
Shigella infection (shigellosis) is a type of gastroenteritis caused by Shigella bacteria. The symptoms of Shigella infection include fever, diarrhoea, (sometimes with blood and mucous), vomiting and stomach cramps.
Enterotoxigenic Escherichia coli (ETEC) is a type of Escherichia coli and one of the leading bacterial causes of diarrhoea in the developing world.
Salmonella infection usually results from ingestion of the bacteria from contaminated food, water or hands. Eggs, milk, meat or poultry are particularly high risk foods.
Shiga toxin-producing Escherichia coli (STEC) strains are human pathogens linked to haemorrhagic colitis and haemolytic uremic syndrome.
Cholera is a well-known disease caused by intestinal infection with the toxin-producing bacteria. This potentially fatal diarrhoeal disease results in large volumes of watery stool, causing rapid dehydration.
Yersinia infection (yersiniosis) is a bacterial infection of the bowel (intestine). It occurs worldwide, but is fairly uncommon. Many domesticated and wild animals carry Yersinia in their intestines, and spread to people occurs by eating food or water contaminated by infected faeces.
Helicobacter Pylori
H. pylori is a type of bacteria that infects the stomach lining and is associated with various gastrointestinal conditions, including gastritis, peptic ulcers, and stomach cancer.
Viral Pathogens
Viral stool pathogens are types of viruses that can cause gastrointestinal infections and are shed in the stool. These viruses can be transmitted through contaminated food, water, or direct contact with an infected individual.
Adenovirus serotypes 40 and 41 cause acute gastroenteritis primarily in children. Symptoms may include fever, diarrhoea, vomiting, and abdominal pain, and last for approximately 10 days.
Norovirus infection can cause the sudden onset of severe vomiting and diarrhoea. The virus is highly contagious and commonly spread through food or water that is contaminated during preparation or through contaminated surfaces.
Astrovirus infections are a common cause of gastroenteritis. The virus spreads through the faecal-oral route, primarily via contaminated food or water, and can also be transmitted through close contact with infected individuals.
Normal Bacterial Gut Flora
Normal bacterial gut flora, also known as gut microbiota or gut microbiome, refers to the diverse community of bacteria that naturally inhabit the gastrointestinal tract of a healthy individual. These bacteria play crucial roles in digestion, metabolism, immune function, and overall health.
Bacteroides fragilis is a species of bacteria that is a normal part of the human gut microbiota. It plays a crucial role in maintaining gut health and has been associated with various beneficial effects.
Bifidobacteria are considered "friendly” bacteria that are found in fermented foods like yogurt and cheese, and are used in probiotics. The gut needs these bacteria to perform several jobs, including breaking down foods, taking in nutrients, and preventing overgrowth of "bad” pathogenic bacteria.
Bifidobacteria are considered "friendly” bacteria that are found in fermented foods like yogurt and cheese, and are used in probiotics. The gut needs these bacteria to perform several jobs, including breaking down foods, taking in nutrients, and preventing overgrowth of "bad” pathogenic bacteria.
Bifidobacteria are considered "friendly” bacteria that are found in fermented foods like yogurt and cheese, and are used in probiotics. The gut needs these bacteria to perform several jobs, including breaking down foods, taking in nutrients, and preventing overgrowth of "bad” pathogenic bacteria.
Bifidobacteria are considered "friendly” bacteria that are found in fermented foods like yogurt and cheese, and are used in probiotics. The gut needs these bacteria to perform several jobs, including breaking down foods, taking in nutrients, and preventing overgrowth of "bad” pathogenic bacteria.
Enterococcus species are part of normal flora in the human gut, but can however be implicated in a variety of infections of which urinary tract infections are the most common.
Most Escherichia strains are harmless and play an essential role in keeping the digestive system healthy, helping to digest food and producing Vitamin K. However, some E. coli bacteria are pathogenic and can cause disease.
Lactobacilli and Bifidobacteria are essential for gut health because they prevent overgrowth of gut pathogens, and contribute to managing intestinal pH, cholesterol, and synthesis of vitamins and disaccharidase enzymes.
Lactobacilli and Bifidobacteria are essential for gut health because they prevent overgrowth of gut pathogens, and contribute to managing intestinal pH, cholesterol, and synthesis of vitamins and disaccharidase enzymes.
Lactobacilli and Bifidobacteria are essential for gut health because they prevent overgrowth of gut pathogens, and contribute to managing intestinal pH, cholesterol, and synthesis of vitamins and disaccharidase enzymes.
Lactobacilli and Bifidobacteria are essential for gut health because they prevent overgrowth of gut pathogens, and contribute to managing intestinal pH, cholesterol, and synthesis of vitamins and disaccharidase enzymes.
Lactobacilli and Bifidobacteria are essential for gut health because they prevent overgrowth of gut pathogens, and contribute to managing intestinal pH, cholesterol, and synthesis of vitamins and disaccharidase enzymes.
Lactobacilli and Bifidobacteria are essential for gut health because they prevent overgrowth of gut pathogens, and contribute to managing intestinal pH, cholesterol, and synthesis of vitamins and disaccharidase enzymes.
While some Clostridium species are associated with severe infections, many Clostridium species are harmless and play important roles in the human gut microbiota.
There is some evidence to suggest that this bacterium lowers the risk of developing kidney stones by breaking down oxalate in the gut.
Akkermansia lives in the mucus lining of your gut and uses mucous as its primary energy source. It regulates mucous turnover and promotes healthy intestinal barrier and modulates immune responses.
A beneficial bacterium that produces butyrate and is associated with gut health maintenance, modulation of immune responses, and potential protection against certain gastrointestinal and metabolic disorders.
Test instructions
Your test kit and all instructions are posted directly to you, and there is no need to visit a collection centre.
Mail your sample back to the lab using the prepaid envelope and packaging.
Results for this test typically available in 2 weeks and will be published in your online dashboard.
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